RNA Sequencing & Biomarker Discovery

RNA is the other major nucleic acid besides DNA. RNA is unique in being able to store and transmit the information as well as process that information. Classically RNAs can be classified into messenger RNAs (mRNAs) and non-coding RNAs (ncRNAs). RNA biomarkers allow for a very early analysis of the appearance or evolution of diseases, a differential analysis of gene expression, an informative and accurate analysis, a specific analysis of the response to drugs.

Accelerate Research and Practice in Biomarker Research

Many kinds of RNAs have been used as biomarkers, among which mRNA is the most widely studied. In recent years, many important functional RNAs that do not encode protein have been discovered, some of which have also been used as biomarkers, including some microRNAs (miRNAs), piwi-separated RNAs (piRNAs), small nucleolar RNAs (snoRNAs), long noncoding RNAs (IncRNAs) and circular RNAs (circRNAs). ncRNAs are very stable molecules, which represents a very important prerequisite for an excellent biomarker. In addition, RNA sequencing analysis can be used for studying regular variation events, such as alternative splicing and gene fusion, both of which may be associated with disease prognosis.

Based on our next-generation sequencing and long-read sequencing platforms, CD Genomics provides a complete solution for biomarker research. Our one-stop service can help you purify high-quality nucleic acids from a variety of research sample types, and use illumina next-generation sequencing, 10x Genomics single-cell sequencing, PacBio sequencing, and Nanopore direct RNA sequencing to provide accurate sequencing results. After bioinformatics analysis, Finally, provide customized reports and serve your scientific research work.

Our advantages
An abundance of sample handling experience
Enrichment and extraction of low-abundance RNAs
Multiple sequencing platforms to choose from
High accuracy of sequencing results
Comprehensive bioinformatics analysis and customized analysis

The issues that can be addressed with our RNA sequencing services
Genetic and epigenetic mechanisms of gene regulation
Disease diagnosis and prognosis
Drug resistance research
Toxicity profiling with transcript analysis
Decoding genomic information for drug discovery

Note: Our services are for research use only, not for disease diagnosis and treatment.

What can we do?

Sequencing targets: mRNA, tRNA, miRNAs, piRNA, snoRNA, IncRNA, circRNA
Sample types: various sample types such as urine, saliva, serum or blood, and tissues
Data delivery: identification, quantitation, differential expression analysis, and other bioinformatics analyses

Service Workflow

Service Workflow

Sequencing Platforms

Illumina NextSeq/HiSeq/NovaSeq 10x Genomics Single-Cell Sequencing PacBio RS Il / Sequel Nanopore
Biomarker gene discovery
Biomarker gene expression
Splice variation
Single-cell biomarker gene discovery
Single-cell biomarker gene expression
Single-cell genetic structure
Biomarker cell heterogeneity
Biomarker gene expression
Biomarker transcript structures
Biomarker gene discovery
Splicing / alternative splicing
Transcription initiation
RNA cleavage / adenylation
Biomarker gene expression
Biomarker transcript structures
Biomarker gene discovery
Splicing / alternative splicing
Transcription initiation
RNA cleavage / adenylation
RNA modification

Analysis Subject

ANALYSIS SUBJECT DETAILS
QC and Visualization Filtering, trimming, optional error correction if possible, and visualization
Transcriptome Assembly Mapping to known transcriptomes; de novo assembly and annotation; outcome evaluation; Transcript and isoform quantification
Functional Profiling GO, DAVID, KEGG, TrEMBL, Pfam, InterPro, SignalP, Blast2GO; Molecular functions or pathways in which differentially expressed genes
Other advanced Analysis In-depth and advanced analyses: Gene fusion analysis, single nucleotide variant analysis, differential transcript expression, ncRNA-gene interaction network

Comprehensive Solutions to Accelerate Your Biomarker Research

CD Genomics has a professional technical team and bio-analysis team, and has more than ten years of project experience in the sequencing field. We have a mature technical route, provide accurate and reliable data and deliver results that meet your requirements. We are committed to providing efficient, cost-effective, and high-quality sequencing services to help your research of biomarkers.

* For Research Use Only. Not for use in diagnostic procedures.


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