Blood RNA Sequencing

CD Genomics offers comprehensive blood RNA sequencing services to characterize total RNA in blood samples, including long non-coding RNA (lncRNA), circle RNA, mRNA and small RNA. Our advanced whole blood transcriptional profiling solution ensures reproducible, specific, sensitive and accurate results to aid disease research, biomarker discovery and other studies.

Overview

Blood is a primary conduit of the individual, involved in systemic immune and metabolic pathways. Peripheral blood cells share over 80% of their transcriptome with many different tissues, including brain, colon, heart, kidney, liver, lung and stomach. Moreover, peripheral blood is the most readily available clinical sample; therefore, blood transcriptome sequencing analysis is uniquely positioned to reveal gene expression signatures, biomarkers and drug responses associated with systemic disease and local pathological activity, such as cancer, hematological tumors, and rare diseases.

In peripheral blood, rRNA content accounts for more than 90% of all RNA, and globin mRNA represents approximately two-thirds of whole blood RNAs. These RNAs can provide little valid information, and direct use of blood RNA samples for sequencing not only generates a large amount of worthless data, but also affects the detection of low-abundance RNAs.

Therefore, we have successfully established a series of practical sequencing and bioinformatics workflows for low load volumes and low-quality blood samples, to significantly improve the detection rate of valid data information and low-abundance RNAs. Combined with specialized next-generation sequencing (NGS), PacBio SMRT and Nanopore sequencing platforms, we accelerate your sequencing and analysis projects to discover and validate novel transcripts, gene fusions, gene expression variants, transcript variants, and gene diversity, and to identify multiple candidate genes and key pathways. Our bioinformatics services are flexible and can be tailored to meet your research needs.

Features

Rich Experience Transcriptome Wide Bioinformatics Analysis One-Stop Solution
High sensitivity and accuracy of low abundance transcript detection. Profile all small RNAs and long RNAs, either known or unknown in the blood transcriptome. Our integrated bioinformatics pipeline can be tailored to suit your project. From sample QC, library construction, to sequencing and data analysis.

Project Workflow

Sample Preparation

1. Sample Preparation

Quality assessment and quantification; ribosomal RNA and globin mRNA depletion

Library Preparation

2. Library Preparation

Size fractionation selection; 18~40bp insert cDNA Library; 250~300bp insert cDNA Library.

Sequencing

3. Sequencing

Illumina Nextseq/HiSeq;
SE50, 7-10 Million Reads;
PE150, >80 Million Reads.

Data Analysis

4. Data Analysis & Delivery

Visualize and preprocess results, and perform custom bioinformatics analysis.

Bioinformatics Analysis Pipeline

Blood RNA Sequencing

In-depth data analysis:

  • Profiling of lncRNA, circRNA, mRNA, siRNA, snoRNA and miRNA
  • Detection of novel and rare transcript variants
  • Differential expression analysis of genes or targets
  • Target gene prediction and functional analysis of lncRNAs and circRNA
  • GO analysis
  • KEGG analysis
  • Customed bioinformatics tertiary analysis

Sample Requirements

Blood sample (≥ 0.5 ml, anticoagulation tubes: sodium citrate or EDTA tubes, heparin tubes are not recommended)

Plasma/serum sample (≥ 2.5 ml with TRIzol LS, anticoagulation tube: sodium citrate or EDTA tube, heparin tube is not recommended)

RNA sample (concentration ≥ 20 ng/uL, amount ≥ 2 ug)

1.8 ≤ OD260/280 ≤ 2.2, OD260/230≥2.0, RIN ≥ 6.5, 28S/18S ≥1.0.

Please make sure that the RNA is not degraded.

Sample Storage: RNA can be dissolved in ethanol or RNA-free ultra-pure water and stored at -80°C. RNA should avoid repeated freezing and thawing.

Shipping Method: When shipping RNA samples, the RNA sample is stored in a 1.5 mL Eppendorf tube, sealed with a sealing film. Shipments are generally recommended to contain 5-10 pounds of dry ice per 24 hours.

Deliverable: FastQ, BAM, coverage summary, QC report, custom bioinformatics analysis.

References:

  1. Harrington CA, Fei SS, Minnier J, et al. RNA-Seq of human whole blood: Evaluation of globin RNA depletion on Ribo-Zero library method. Scientific reports, 2020, 10(1): 1-12.
  2. Paskey AC, Frey KG, Schroth G, et al. Enrichment post-library preparation enhances the sensitivity of high-throughput sequencing-based detection and characterization of viruses from complex samples. BMC genomics, 2019, 20(1): 1-14.
* For Research Use Only. Not for use in diagnostic procedures.


Inquiry

  • Verification code
logo
Copyright © 2022 CD Genomics. All rights reserved.