Our service is designed to provide researchers with a comprehensive understanding of long non-coding RNAs (LncRNAs) and circular RNAs (CircRNAs) and their interactions with RNA-binding proteins, based on our RIP Sequencing platform.
LncRNAs and CircRNAs are two fascinating classes of non-coding RNAs that have gained immense attention due to their regulatory roles in gene expression and cellular processes.
LncRNAs, although not encoding proteins, wield remarkable influence over cellular function. They are lengthy RNA molecules that orchestrate a symphony of gene regulatory activities. By interacting with chromatin-modifying enzymes and recruiting them to specific genomic regions, LncRNAs sculpt the landscape of DNA and histone modifications. This dynamic choreography affects chromatin structure, accessibility, and ultimately gene expression. As molecular architects, LncRNAs guide the cell in adapting genomic functions to developmental cues and environmental signals, ushering genes into appropriate expression patterns.
CircRNAs are marked by their unique closed-loop structure, distinct from the linear form of most RNA molecules. This distinctive topology enables them to engage in intricate intracellular regulatory networks. While the full spectrum of CircRNA functions is still being uncovered, it's evident that they serve as essential participants in fine-tuning cellular processes. Their potential roles in sponging microRNAs, interacting with RNA-binding proteins, and influencing translation make them intriguing candidates for a wide range of regulatory tasks.
RNA Immunoprecipitation (RIP) sequencing is a powerful technique that allows researchers to investigate the interactions between RNA molecules and RNA-binding proteins (RBPs). By isolating the complexes formed between LncRNAs/CircRNAs and RBPs, we can decipher the regulatory roles of these non-coding RNAs and shed light on the intricate mechanisms governing gene expression.
Workflow of RIP Sequencing – CD Genomics
|Enable the identification of even the most delicate protein binding sites across the entire genome.
|Capture intricate details and reveal the subtleties of RNA interactions that might otherwise remain hidden.
|Enable you to explore interactions, pathways, and networks across multiple layers, uncovering the interplay between RNAs and proteins.
|One-stop solution, covering everything from library construction and sequencing to sample quality control and data analysis.
For sample volumes ≥ 200 ng: Our standard library preparation procedures will be employed.
For sample volumes ≤ 10 ng: Our library preparation approach will be utilized to accommodate lower input amounts without compromising data quality.
Tissue Samples: A minimum of ≥ 100 mg of tissue is required for optimal results.
Cell Samples: Cell samples must not be fewer than 107 cells.
Mycoplasma Contamination: Cell samples must be free of mycoplasma contamination to ensure reliable results.
Sample Storage: RNA can be dissolved in ethanol or RNA-free ultra-pure water and stored at -80°C. RNA should avoid repeated freezing and thawing.
Shipping Method: When shipping RNA samples, the RNA sample is stored in a 1.5 mL Eppendorf tube, sealed with sealing film. Shipments are generally recommended to contain 5-10 pounds of dry ice per 24 hours.
Deliverable: Comprehensive RIP experiment report. Fastq, BAM, coverage summary, QC report, custom bioinformatics analysis.