RNA microarray is a mature and reliable high-throughput experimental screening tool, and it is the tool of choice for ceRNA research. CD Genomics has many years of microarray R&D and service experience, providing researchers with complete lncRNA, circRNA and mRNA screening.
Overview
Post-transcriptional regulation of genes is a very complex and fine regulatory network. Among them, the regulatory mechanism of competing endogenous RNA (ceRNA) is the most interesting: microRNA (miRNA) is at the center of this regulatory network. Since long non-coding RNA (lncRNA) molecules are usually rich in miRNA-binding sites, they can bind miRNA competitively, relieve the inhibitory effect of miRNA on its target genes, and then regulate the expression level of genes. In the ceRNA mechanism, circRNAs and lncRNAs are typical ceRNAs because they often carry multiple miRNA binding sites. Elucidating the ceRNA regulatory mechanism of circRNA and lncRNA will help researchers to deeply understand the fine regulatory network in the occurrence and development of disease, and may guide drug development and disease diagnosis and treatment.
circRNAs and lncRNAs are canonical ceRNA mechanisms. CD Genomics' ceRNA microarray probes are 60mer long. Our ceRNA microarray has a high detection rate, technical repeatability higher than 99%, good stability, and covers circRNA, lncRNA, and mRNA. It can be more sensitive and stable than high-throughput sequencing technology. circRNA and lncRNA of low abundance can be detected and analyzed.
Features
High Sensitivity & High Precision
High Specificity
Comprehensive Annotations
Professional Bioinformatics
Can detect low expression of single copy in each cell.
Avoid rRNA or exogenous sequence interference.
Comprehensive ceRNA targets and provides sufficient ceRNA biological function annotations.
High quality lncRNA database, systematic and detailed annotation and functional analysis.
Project Workflow
1. Sample Preparation
RNA purification; quality assessment and quantification.
2. Library Preparation
cDNA library construction, labeling, and labeling efficiency quality assessment.
3. Microarray Hybridization
The labeled probe was hybridized with a high-density microarray under standard conditions.
4. Data Analysis
Preprocess and visualize results, and perform custom bioinformatics analysis.
Bioinformatics Analysis Pipeline
In-depth data analysis:
We can provide detailed circRNA-miRNA, mRNA-miRNA, lncRNA-miRNA, and mRNA-miRNA-ncRNA interaction annotations, which will help researchers to further explore the functional role of ceRNAs and differential expression analysis.
Sample Requirements
RNA samples or well-preserved tissue/cell specimens; RNA≥200 ng; Species: human, mouse, rat
Sample storage: Cell samples or fresh tissue pieces (cut into 5-10 mg pieces) can be treated with TRIzol or RNA protectant, frozen in liquid nitrogen, and stored at -80°C. RNA samples can be dissolved in ethanol or RNA-free ultrapure water, and stored at -80°C. Avoid repeated freezing and thawing during sample storage.
Shipping Method: The sample is stored in a 1.5 mL Eppendorf tube, sealed with sealing film. Shipments are generally recommended to contain 5-10 pounds of dry ice per 24 hours.
References:
Du L, Wang X, Yin Y, et al. Identification of a potentially functional circRNA-miRNA-mRNA ceRNA regulatory network in bladder cancer by analysis of microarray data. Translational Andrology and Urology. 2021 Jan;10(1).
Wang Z, Xu P, Chen B, et al. Identifying circRNA-associated-ceRNA networks in the hippocampus of Aβ1-42-induced Alzheimer's disease-like rats using microarray analysis. Aging. 2018 Apr;10(4).
* For Research Use Only. Not for use in diagnostic procedures.
