Circulating miRNAs Sequencing (Cell-Free miRNA Seq)

At CD Genomics, we offer a comprehensive range of untargeted circulating miRNA detection and analysis services, utilizing our small RNA next-generation sequencing and data analysis platforms. Our services are designed to enable your extracellular miRNA research and biomarker discovery breakthroughs.


Since 2008, circulating miRNAs have been discovered in various extracellular human body fluids such as serum, plasma, saliva, urine, tears, etc. These circulating miRNAs are encapsulated in small membrane vesicles (e.g., exosomes, microparticles, and apoptotic bodies) or bound to proteins such as HDL. They play a crucial role in intercellular communication, as they are released into the bloodstream upon tissue damage or active secretion, and are transported to target cells to regulate gene expression and impact cellular function.

The stability of circulating miRNAs is a significant advantage. Unlike intracellular miRNAs, miRNAs in biofluids are protected from degradation by RNase enzymes due to their encapsulation or protein binding. They exhibit remarkable stability, allowing for long-term storage at room temperature and resistance to multiple freeze-thaw cycles and extreme pH conditions. Furthermore, circulating miRNAs show low variability and remain relatively stable over the lifespan of healthy individuals, making them potentially valuable as disease biomarkers.

Our team of experts is dedicated to delivering high-quality sequencing data and comprehensive analysis to uncover the potential of circulating miRNAs and exosome miRNAs in disease diagnostics, therapeutic response monitoring, and biomarker discovery.

Are you interested in our Exosome miRNA Sequencing Solution? Take a look at our website to know about cellular health, disease states, and more by analyzing exosomes and extracellular vesicles (EVs).


High Compatibility Efficiency Cutting-edge Algorithms One-Stop Solution
Robust and optimized miRNA extraction and purification process. Minimize PCR bias and quantify miRNA molecules incorporating unique molecular identifiers (UMIs). Correlate miRNA expression patterns with specific diseases or clinical outcomes. One-stop solution from sample QC, library construction, to sequencing and data analysis.

Project Workflow

Sample Preparation

1. Sample Preparation

Library Preparation

2. Library Preparation


3. Sequencing

Data Analysis

4. Data Analysis

Bioinformatics Analysis Pipeline

Bioinformatics Analysis Pipeline

1. Read Alignment and miRNA Annotation

2. Quantification and Normalization

3. Differential Expression Analysis

4. Functional Analysis

5. Biomarker Identification

Case Studies

Sample Requirements

RNA sample (concentration ≥ 200 ng/uL, quantity ≥ 1 ug)

1.8 ≤ OD260/280 ≤ 2.2, OD260/230≥2.0, RIN ≥ 6.5, 28S:18S≥1.0.

Please make sure that the RNA is not degraded.

Sample Storage: RNA can be dissolved in ethanol or RNA-free ultra-pure water and stored at -80°C. RNA should avoid repeated freezing and thawing.

Shipping Method: When shipping RNA samples, the RNA sample is stored in a 1.5 mL Eppendorf tube, sealed with a sealing film. Shipments are generally recommended to contain 5-10 pounds of dry ice per 24 hours.

Deliverable: FastQ, BAM, coverage summary, QC report, custom bioinformatics analysis.


  1. Ali, Shabana A., et al. "Sequencing identifies a distinct signature of circulating microRNAs in early radiographic knee osteoarthritis." Osteoarthritis and Cartilage 28.11 (2020): 1471-1481.
* For Research Use Only. Not for use in diagnostic procedures.

Research Areas
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