Exosomal small RNA sequencing is a next-generation sequencing (NGS)-based method for comprehensive transcriptome analysis of exosomal small RNA species. It can accurately explore the mechanisms underlying gene expression regulation and intercellular communication, and discover biomarkers for disease diagnosis and monitoring. We provide high-throughput sequencing of exosomal small RNAs to quickly and efficiently obtain the global information of exosomal small RNAs.
The exosome is a type of membranous vesicles produced in the endosomal compartment of eukaryotic cells and releases its vesicular cargo into the extracellular space. It is typically 30-100 nm in diameter and contains nucleic acids and proteins. Exosomes serve as transporters of these substances between cells and participate in physiological processes such as immune response, inflammatory response, angiogenesis, apoptosis, coagulation, and waste disposal. It can be used as an early diagnostic marker for diseases and as a carrier for drug delivery and targeted drug therapy. Small RNAs are non-coding RNA (ncRNA) molecules with length less than 200 nucleotides, mainly including microRNA, tRF, tiRNA, piRNA, tRNA, snoRNA and pre-microRNA. As important regulatory molecules, small RNAs are involved in gene expression, epigenetics, and development of diseases. The expression of exosomal small RNA significantly varies in different disease models.
Exosomal small RNA sequencing (RNA-seq) can quickly and efficiently obtain comprehensive information of exosomal small RNAs, enabling target gene analysis and functional analysis. Exosomal small RNA-seq is an ideal method for the discovery of biomarkers as its convenience for identifying the differential expression patterns in disease models. We provide customers with comprehensive exosomal small RNA-seq service and in-depth data analysis for a better understanding of the roles that exosome small RNAs play in gene expression regulation and intercellular communication, so as to identify potential therapeutic targets, early diagnostic biomarkers and targeted drug carriers for multiple diseases.
|High Resolution||Comprehensive Information||Accurate Annotation||Superiority|
|Allow single-base resolution identification of small RNAs.||Detect multiple types of small RNAs at one time, including miRNA, siRNA, piRNA, etc.||Provide complete annotate information with multiple databases for exosomes or vesicles.||Rich experience in experimental design, operations and data analysis.|
Quality assessment and quantification.
Size selection (17 to 35 nt), cDNA library construction, library QC by BioAnalyzer and RTQ-PCR.
Illumina HiSeq; 50/75/100 nt, single or paired-end.
Perform custom bioinformatics analyses and provide detailed data report.
RNA sample quantity ≥ 2 ng, volume ≥ 20ul.
1.8 ≤ OD260/280 ≤ 2.2, OD260/230 ≥ 2.0, RIN ≥ 6.5, 28S:18S ≥ 1.0.
Please make sure that the RNA is not significantly degraded.
Sample storage: RNA can be dissolved in ethanol or RNA-free ultra-pure water and stored at -80°C. RNA should avoid repeated freezing and thawing.
Shipping Method: When shipping RNA samples, the RNA sample is stored in a 1.5 mL Eppendorf tube, sealed with sealing film. Shipments are generally recommended to contain 5-10 pounds of dry ice per 24 hours.
Deliverable: FastQ, BAM, coverage summary, QC report, custom bioinformatics analysis.