High-throughput RNA sequencing can obtain a large amount of transcript sequence information, covering a large amount of coding and non-coding RNAs. The value of RNA sequencing in microbiological research includes microbial identification, microbial resistance analysis, microbial diversity analysis, microbial gene expression analysis and more. Through the analysis of genome sequence splicing annotation, functional gene analysis, and gene transcription differential expression, microbial RNA sequencing and analysis can be widely used in research in many fields such as human health, plant-microbe interaction, animal-microbe interaction, ecological environment, fermentation, drug research and development.

Accelerate Research and Practice in Microbiology

CD Genomics offers a complete solution for microbial RNA sequencing. Our one-stop service starts from assisting in design, to conducting experiments and finally submitting satisfactory reports. We have multiple sequencing platforms to address your research needs, including Illumina next-generation sequencing, PacBio SMRT, Nanopore sequencing, and single-cell sequencing. We can also provide customized bioinformatics analysis and provide detailed reports.

CD Genomics can support all aspects of your research, including but not limited to microbial identification (bacteria, fungi, viruses, etc.), transcriptome research, microbial gene regulation, and drug resistance research. Our thoughtful and meticulous service can help your research discovery and research achievement transformation in many fields.

Our advantages
Flexible to various of research fields
Enrichment and extraction of low-abundance targets
Multiple sequencing platforms to choose from
High accuracy of sequencing results
Comprehensive bioinformatics analysis and customized analysis

The issue that can be addressed with our microbial RNA sequencing services
Fundamental research of microbiology
Human disease and health research
Research on the Interaction of plants and microorganisms
Research on the interaction between animals and microorganisms
Ecology and environmental research
Industrial fermentation
Drug research and development

Note: Our services are for research use only, not for disease diagnosis and treatment.

What can we do?

Sample requirement: a. animal tissues, plant tissues, or environmental samples: fresh samples, sample amount ≥ 2 g. (note: after sampling, quick-freeze in liquid nitrogen and store at -80°C. Use dry ice for transportation when sending samples).

b. Total RNA: concentration ≥ 250 ng/μl, amount ≥ 50 μg; OD_260/280=1.8-2.2, OD_260/230 ≥ 2.0. 28S:18S > 1.5; RIN > 8.0; and ensure that the RNA is not degraded and without pollution; or provide mRNA with concentration ≥ 50 ng/μl and total amount > 1 μg

Data delivery: identification, quantitation, evolution and function analysis, and other bioinformatics analyses

Service Workflow

Sequencing Platforms

Illumina NextSeq/HiSeq/NovaSeq	10x Genomics Single-Cell Sequencing	PacBio RS IL / Sequel	Nanopore
Microbial gene discovery Microbial gene expression Splice variation	Single-cell microbial gene discovery Single-cell microbial gene expression Single-cell genetic structure	Microbial gene expression Microbial transcript structures Microbial gene discovery Splicing / alternative splicing Transcription initiation RNA cleavage / adenylation	Microbial gene expression Microbial transcript structures Microbial gene discovery Splicing / alternative splicing Transcription initiation RNA cleavage / adenylation RNA modification

Bioinformatics Analysis Contents

ANALYSIS CONTENTS	DETAILS
Read QC	Raw data sorting, filtering and quality assessment
Transcriptome Assembly	Mapping to known transcriptomes; de novo assembly and annotation to protein database; outcome evaluation; Transcript and isoform quantification
Functional Profiling	Function annotation Functional cluster analysis (KOG, GO) Metabolic pathway analysis (KEGG pathway) Differential expression analysis Cluster analysis of differential expression (heat map) Differential expression enrichment analysis SNP identification Variable shear analysis Novel transcript analysis …
Advanced Analysis	Detection of eQTL/sQTL Chromatin TF binding Analysis of integration with other technologies …

Comprehensive Solutions to Accelerate Your Microbiology Research

CD Genomics has a professional technical team and bioinformatics analysis team, as well as more than ten years of project experience in the sequencing field. We have optimized to a mature technical route, providing accurate and reliable data and high-quality results that meet your requirements. We are committed to providing efficient, cost-effective, and satisfying sequencing services to help your research in microbiology.