As a mature technology platform, DNA/RNA microarray has been widely used in the detection of gene expression. For lncRNA expression profiling, RNA microarrays have many important and irreplaceable advantages over RNA sequencing: first, the accuracy of microarray detection is less affected by low-abundance transcripts; second, lncRNAs do not have the translation start and end sites of proteins, and the reliability of identification of lncRNA transcripts is lower than that of mRNAs for RNA sequencing. While the microarray probes are shorter and can be specifically designed, and the detection is more specific.
Overview
Long non-coding RNAs (lncRNAs) are a class of RNAs with a length of more than 200 nt. They do not encode proteins themselves but are regulate gene expression at various levels (epigenetic regulation, transcriptional regulation, post-transcriptional regulation, etc.) in the form of RNA. LncRNAs are generally expressed and can function at low levels. Recent studies have shown that lncRNA is widely involved in various biological processes, and the abnormal expression of lncRNA is closely related to many diseases including cancer. Through the lncRNA microarray, researchers can quickly obtain the expression changes of lncRNAs related to specific biological processes or diseases in a high-throughput manner, thus providing great convenience for subsequent functional research or biomarker screening.
CD Genomics' RNA microarray can detect lncRNA and mRNA simultaneously. Our lncRNA microarray fully supports human, mouse, rat, and covers the full-length information of lncRNA, enabling accurate and rich annotation. Our lncRNA microarray includes detailed and systematic lncRNA annotation, classification, and analysis to gain insight into the complex biological functions of lncRNAs. LncRNAs that have been reported to be related to biological processes or human diseases are being studied, annotated, and cross-referenced, from which rich sources of information can help to reveal the functional roles and molecular mechanisms of lncRNAs.
Features
High Sensitivity & High Precision
High Specificity
Transcript Isomer
Professional Bioinformatics
Quantitative lncRNAs detection with high sensitivity can detect even single lncRNA copy in each cell.
Naturally capable of chain specificity detection for both sense and anti-sense lncRNA.
LncRNA transcript isomers can be detected explicitly and specifically.
High-quality lncRNA database, systematic and detailed annotation and functional analysis.
Project Workflow
1. Sample Preparation
RNA purification; quality assessment and quantification.
2. Library Preparation
cDNA library construction, labeling, and labeling efficiency quality assessment.
3. Microarray Hybridization
The labeled probe was hybridized with a high-density microarray under standard conditions.
4. Data Analysis
Preprocess and visualize results, and perform custom bioinformatics analysis.
Bioinformatics Analysis Pipeline
In-depth data analysis:
We can provide data reports including but not limited to microarray scans, RNA quality reports, microarray data result reports, differential lncRNA expression lists, differential gene expression lists. Our one-stop microarray analysis contains systematic, detailed and professional lncRNA annotations such as genomic information, epigenomic information, subclass analysis, transcript sequence integrity, subcellular localization, miRNA recognition sites, species conservation, tissue/cell specificity and short peptide coding potential, related biological processes, and disease correlations, help you gain an in-depth understanding of the biological functions and molecular mechanisms of lncRNAs.
Sample Requirements
RNA samples or well-preserved tissue/cell specimens; RNA≥200 ng; Species: human, mouse, rat
Sample storage: Cell samples or fresh tissue pieces (cut into 5-10 mg pieces) can be treated with TRIzol or RNA protectant, frozen in liquid nitrogen, and stored at -80°C. RNA samples can be dissolved in ethanol or RNA-free ultrapure water, and stored at -80°C. Avoid repeated freezing and thawing during sample storage.
Shipping Method: The sample is stored in a 1.5 mL Eppendorf tube, sealed with sealing film. Shipments are generally recommended to contain 5-10 pounds of dry ice per 24 hours.
References:
Zhang K, Shi H, Xi H, et al. Genome-wide lncRNA microarray profiling identifies novel circulating lncRNAs for detection of gastric cancer. Theranostics. 2017;7(1).
Chen J, Fu Z, Ji C, et al. Systematic gene microarray analysis of the lncRNA expression profiles in human uterine cervix carcinoma. Biomedicine & pharmacotherapy. 2015 May 1;72.
* For Research Use Only. Not for use in diagnostic procedures.
