At CD Genomics, we not only offer a wide range of efficient sequencing platforms, but also powerful bioinformatic analysis capabilities that have led to breakthroughs in academic and clinical research. We are committed to empowering RNA research by deciphering the genetic code, including areas such as disease genesis, drug discovery, and biomarker identification.
At CD Genomics, we understand the significance of choosing the right sequencing platform for your research needs. Short-read sequencing platforms offer several key advantages, making them highly versatile for a wide range of applications.
Short-read sequencing platforms (especially Illumina) provide a large volume of data, typically yielding an average of 20-30 million reads per sample, quantification of each gene or transcript, and then statistical analysis of differential genes. Many genes with low expression abundance can be sequenced. Single-cell sequencing also mainly adopts short-read sequencing due to the large amount of data.
MiSeq
NextSeq 500
NovaSeq 6000
Hiseq X Ten
Platform
Highlights
More than 1Gb of data per run
Sequencing throughput is at least 4x greater than the MiSeq, making it easy to sequence large-volume data, and long fragment samples
Increase your sample volume and coverage by seamlessly mixing and matching four types of flow-through tanks.
Process tens of thousands of samples and achieve a minimum 30x coverage for human genome sequencing.
Scalable and flexible data output, supporting expression profiling, whole genome sequencing (WGS), ultra-deep targeted sequencing, and tumor profiling.
Variant detection to facilitate the construction of population-scale reference data.
Run Time
4–24 hours
11-48 h
~13–38 h (dual SP flow cells)
~13–25 h (dual S1 flow cells)
~16–36 h (dual S2 flow cells)
~44 h (dual S4 flow cells)
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Maximum Output
7.5 Gb
360 Gb
6000 Gb
1.6–1.8 Tb
Maximum Read Length
2 × 150 bp
2 × 150 bp
2 x 250 bp
2 × 150 bp
Long-Read Sequencing Platforms
Long-read sequencing platforms (including PacBio SMRT and Nanopore sequencing) have this absolute advantage in alternative splicing, gene fusion, RNA methylation and so on.
ONT MinION
ONT PromethION
PacBio Sequel II
Platforms
Highlights
Direct sequencing of individual molecules without the need for amplification.
Overcome issues related to PCR amplification preferences and GC content biases.
Navigate seamlessly through regions with abnormal GC content, whether too high or too low, and effectively manage high sequence duplications.
Attain complete and uniform sequence coverage, mitigating challenges associated with splicing problems.
Improve the quality and depth of reference genome annotation information.
Detect various RNA modifications, including methylation, 5hC, 5hmU, 5hU, 1mA, 6mA, 8oxoA, BPDE, 6mT, 6mG, and more.
Accuracy
96–99%
88–90/99.9% (CCS)
Runtime
10-30 h
72 h
Average Output
5x20 Gb per run
48x50 Gb per run
160 G
Maximum Read Length
10-100 kb, up to 4 Mb
10-15 kb, up to 20 kb
CD Genomics Full-length transcriptome sequencing workflow
Single-Cell and Spatial Transcriptome Platforms
10X Genomics
10X Genomics use microfluidics, oil droplet encapsulation, and barcode labeling to capture and analyze 500-10,000 single cells at once. This technology is cost-effective, has a short capture time, and is used for cell typing, identifying marker genes, and studying gene expression differences. It's essential in disease, immunity, oncology research, tissue and organ studies, and understanding cell differentiation and developmental trajectories.
CD Genomics Full-length transcriptome sequencing workflow
