Transcriptional Start Site (TSS) Analysis
Transcriptional Start Site (TSS) selection influences transcriptional stability and diversity in development and disease. CD Genomics offers cutting-edge capture-based sequencing solutions for comprehensive genome-wide TSS analysis.
Overview
Gene expression is primarily regulated through transcriptional processes, where cis-acting promoters interact with the transcription machinery. The Transcription Start Site (TSS) marks the location of the first base where a gene is transcribed from DNA into RNA. Different TSS locations result in genes with distinct 5'UTRs, impacting their regulation. Most genes have multiple TSSs, and identifying mRNA TSSs is crucial for understanding gene regulation.
Advances in sequencing technology have made genetic data-dependent TSS prediction more accessible, but accuracy and refinement are needed. Different TSSs may be chosen based on environmental factors, even within the same species and gene, leading to varied transcription start site shapes. Accurate transcription initiation is essential for proper gene expression, and dysregulation can contribute to various human diseases, including breast cancer, diabetes, kidney failure, and Alzheimer's disease.
Features
- Precision & Accuracy: Our state-of-the-art sequencing methods deliver unparalleled precision and accuracy in TSS identification, down to the single base level.
- Comprehensive Genome-Wide Coverage: Explore TSS across entire genomes with confidence. Our services provide comprehensive genome-wide coverage for your target species.
- Customized Solutions: Collaborate with our experts to tailor our TSS analysis to your unique research needs. We're here to help you achieve your scientific goals.
- Expert Support: Benefit from our dedicated team of experts who guide you from experimental design to data analysis and interpretation.
Service Portfolio
Explore how we efficiently, rapidly, and accurately identify genome-wide transcription start sites (TSS) through sequencing.
CAGE-seq
CAGE-seq (cap analysis of gene expression-sequencing) allows for the genome-wide identification of transcription start sites and determination of the mRNA's originating promoter. This technique involves large-scale sequencing and tagging of the 5' end of cDNAs.
Learn MoreCappable-Seq
Cappable-seq directly modifies and characterizes triphosphorylated RNA from primary transcripts. It provides global transcription start site (TSS) identification with single-base resolution and is compatible with various sequencing platforms, including NGS and long-read sequencing.
Learn MoretagRNA-Seq
Utilizing short artificial RNA tags, tagRNA-seq distinguishes between primary and processed transcripts. It offers a detailed view of the bacterial RNA landscape, including TSS, PSS, and asRNA.
Learn MorePacBio Iso-Seq
PacBio Iso-Seq technology provides unique long-read capability for sequencing large amplicons and whole transcripts with extraordinary precision to identify changes in transcription start sites (TSS) associated with critical traits.
Learn MoreApplications of TSS Analysis
- Studying Transcriptional Regulation: Uncover key regulatory elements, promoter regions, and transcription factors influencing gene expression.
- Disease Mechanisms: Investigate how TSS variations contribute to disease development, aiding in the identification of potential therapeutic targets.
- Drug Discovery: Identify novel drug targets and pathways by understanding the TSS dynamics of relevant genes.
- Biomarker Discovery: Discover TSS-associated biomarkers for disease diagnosis, prognosis, and treatment response.
Sequencing Platforms
- Illumina HiSeq 2500 / HiSeq 4000 / HiSeq X Ten / NovaSeq 6000 / NextSeq 500 / MiSeq
- PacBio RS II / Sequel
- Nanopore MinION / GridION
- 10X Genomics
