Gene Expression Profiling by RNA Sequencing

Leveraging high-throughput sequencing and microarray technologies, our gene expression profiling service empowers scientists to delve deep into the intricate world of genetic activity across diverse sample types. We facilitate the identification of the molecular underpinnings behind phenotypic variations, guiding researchers in selecting key gene expression targets for further exploration through various methodologies.

Features

• Applicable to a diverse range of species, spanning from microorganisms to humans, ensuring adaptability to various research needs.
• Extensive experience in both next-generation and long-read sequencing data analysis.
• Profile all types of RNA, including mRNAs, lncRNAs, circRNAs, and microRNAs, whether they are known or unknown.
• Data analysis solutions that go beyond RNA-Seq to provide a fresh perspective for you.

Service Portfolio

• • Sequencing Solutions
    • • Gene Expression Profiling by Sequencing: Harness the power of transcriptome sequencing using next-generation sequencing for a neutral and reliable method of uncovering expressed gene differences.
      • Whole Transcriptome RNA-Seq: Explore the entire transcriptome, gaining a holistic view of gene expression patterns.
      • Targeted RNA Sequencing: Zoom in on specific RNA sequences, allowing for a focused analysis of particular genes of interest.
      • Small RNA Sequencing: Delve into the realm of small RNA molecules, capturing nuanced expressions that other methods might overlook.
      • Long Read RNA Sequencing: Full-length RNA sequencing with PacBio Iso-Seq and Nanopore allows for comprehensive insights of the transcriptome, paving the way for the characterization of complex alternative splicing, fusion transcripts and gene expression regulations.

      Learn more about our RNA Sequencing Solutions.

  • Microarray Excellence for Comprehensive Profiling
    • • Gene Expression Profiling by Microarray: Our RNA microarrays, enriched with the latest content from public sequence data sources, facilitate swift and detailed whole-transcriptome expression profiling.
      • LncRNA Microarray: Specialized in detecting genes, long-stranded non-coding RNAs, exons, and splice variants, providing a nuanced analysis of genetic elements.
      • Reliable Profiling: Our microarray tests ensure the accurate profiling of well-annotated genes, even with challenging samples that may lack the necessary bioinformatic resources.
  • High-Throughput Gene Expression Screening

    • Based on High-Throughput Sequencing equipped with Unique Molecular Identifiers (UMI), we offer a cutting-edge solution for rapid, large-scale detection of gene expression. This innovative platform not only expedites the process of target-based drug discovery and screening but also optimizes efficiency by reducing library preparation time and sequencing costs.

      1. Rapid and Efficient Screening:

      Accelerate gene expression analyses with High-Throughput Sequencing for quick and efficient screening.

      2. Cost-Effective Operations:

      Reduce library preparation time and sequencing costs without compromising resolution or sensitivity.

      3. Precision with Unique Molecular Identifiers (UMI):

      Enhance accuracy with UMI, ensuring robust quantification and minimizing biases in your data.

      4. Dynamic Detection of Cellular Changes:

      Capture dynamic changes in cellular gene expression, gaining insights crucial for drug discovery research.

      Learn more about our Gene Expression Screening for Drug Research, Cellular Response Studies, and Treatment Phenotyping.

  • Comprehensive Gene Expression Solutions by Sample Types
    • • Eukaryotic Gene Expression Regulation

      Dive into the intricate world of eukaryotic gene expression regulation, gaining a deep understanding of molecular processes within complex organisms.

      • Prokaryotic Gene Expression Regulation

      Uncover the nuances of prokaryotic gene expression regulation, facilitating insights into the genetic mechanisms of simpler organisms.

      • Tumor Tissue Gene Expression Profiling

      Gain valuable insights into the molecular landscape of tumor tissues, aiding in the identification of key markers and potential therapeutic targets. You may be interested in our FFPE RNA-Seq Service.

      • Single-cell RNA Sequencing

      Elevate your research with the power of single-cell RNA sequencing, unraveling the heterogeneity within cellular populations for precise gene expression analysis.

      • Crop Expression Profiling

      Revolutionize crop research by delving into comprehensive expression profiling, providing essential information for crop improvement and optimization. We also provide single-cell RNA sequencing for plants.

      • Spatial Transcriptome Sequencing

      Explore the spatial dimension of gene expression with our advanced spatial transcriptome solutions, offering a holistic view of gene activity within tissues.

      • Spatio-temporal Expression Profiling

      Unravel the dynamics of gene expression over both space and time, allowing for a nuanced understanding of how genes are regulated under different conditions.

General Service Workflow

Application Fields

• Cancer transcriptomics
• Population genetics
• Drug and biomarker development
• Pharmacogenomics applications
• Agricultural applications

Sequencing Platforms

• Illumian HiSeq X Ten / NovaSeq 6000 / NextSeq 500 / MiSeq
• PacBio Sequel II / Revio
• Nanopore PromethION / MinION
• 10X Genomics
• MGI DNBSEQ-T7 / DNBSEQ-G400

Learn more about our platforms

Sample Requirements

• RNA sample (concentration ≥ 200 ng/uL, quantity ≥ 2 ug). Please refer to our Sample Submission Guideline for more information.
• 1.8 ≤ OD_260/280 ≤ 2.2, RIN ≥ 7.0, 28S:18S ≥ 1.0.
• Please make sure that the RNA is not degraded.

Sample Storage: RNA can be dissolved in ethanol or RNA-free ultra-pure water and stored at -80°C. RNA should avoid repeated freezing and thawing.

Shipping Method: When shipping RNA samples, the RNA sample is stored in a 1.5 mL Eppendorf tube, sealed with sealing film. Shipments are generally recommended to contain 5-10 pounds of dry ice per 24 hours.

Deliverable: FastQ, BAM, coverage summary, QC report, custom bioinformatics analysis report.

FAQ

• • How do you deal with RNA sample contamination?

    • Inhibitors may be present in the sample and affect amplification efficiency. Solutions include sample purification, sample dilution to dilute inhibitors, and the use of unique molecular identifiers (UMIs) to reduce library preparation time and cost.

• • How to deal with RNA degradation?

    • RNA is susceptible to nuclease degradation, especially during extraction and preservation. Solutions include the use of RNase inhibitors, strict control of experimental operation time and temperature, and the use of RNase-free labware.

• • How to solve the problem of difficulty in detecting low abundance genes?

    • There is a subset of genes that are expressed at very low levels in cells, making detection of these low abundance genes difficult. Solutions include the use of unique molecular identifiers (UMIs) or the use of ultra-low sample size and high sensitivity assays to achieve the detection of these genes.

• • How much RNA is needed for detection by Gene Expression Microarray?

    • The minimum total RNA required for detecting both eukaryotic and prokaryotic species using Expression Chip is 20 ng.

• • How to collect and handle samples before submission?

    • Guidelines for sample collection and handling are detailed in our Sample Submission Guide, tailored for various sample types and processing methods. Customers can access this guide on our official website for comprehensive information.

• • What are the required quantities for tissue, cells, or blood samples?

    • The specified amounts are 100 mg for tissue, 10⁶ for cells, and 2 ml for blood. For blood samples, it is advisable to conduct erythrocyte lysis, isolate leukocytes, and add Trizol before submission. All samples should be shipped on dry ice, ensuring suitability even in remote areas. Refer to our Sample Submission Guide.