Non-coding RNA Sequencing
Non-coding RNAs (ncRNAs) are a class of RNA molecules that cannot encode protein products. Many non-coding RNAs used to be considered "junk", but now, researchers have gradually realized their importance in the regulation of gene expression and epigenetic processes such as heterochromatin formation and gene silencing. The well studied non-coding RNAs including miRNAs, lncRNAs, circRNAs, rRNAs, tRNAs, piRNAs, etc. RNA-Seq is a robust tool for transcriptome profiling using next-generation sequencing (NGS) technologies to detect and quantify RNA molecules at a given moment in time. Targeted bioinformatics analysis enables the identification and characterization of ncRNAs from RNA-seq raw data. Alternatively, RNA-seq combined with panels targeting a specific group of ncRNAs enhance the sequencing depth and coverage, simplify bioinformatics analysis, and reduce cost and time.
Non-coding RNA research has gained widespread attention in recent years as many non-coding RNAs are closely associated with development and disease. We provide a full range of non-coding RNA sequencing services to profile non-coding RNAs, examine their changes in the abundance across samples, detect novel non-coding RNA molecules, and reveal the complex networks of interaction with other DNA/RNA/proteins.
- Detect novel transcripts and Discover novel biomarkers
- Examine the sequences and quantity of ncRNA molecules
- A full range of non-coding RNA sequencing solutions to satisfy your specific needs
- Superior data quality: ≥80% bases with ≥Q30, >90% on average
- Comprehensive bioinformatics analysis in an efficient and customizable way
Explore how our ncRNA sequencing services help you understand ncRNA dynamics through transcriptomic and bioinformatic insights.
Long RNA sequencing detects long RNA (>200 nt) including lncRNA, circRNA, and mRNA. We provide long RNA-seq to reveal how RNA molecules affect gene regulation, and thus disease development and phenotypic variation.
circRNA-seq focuses on the sequence and quantity analysis of circular RNAs. We provide this method to revel the pivotal roles of circRNAs in fundamental biological pathways such as miRNA sponges and cap-independent translation.
lncRNA-Seq provides a comprehensive analysis of lncRNAs, enabling the detection of lncRNA expressions under specific conditions or in different tissues and revelation of lncRNA functions.
Small RNA-seq is a powerful tool for analyzing small RNAs such as miRNAs, siRNAs, and piRNAs in a single sequencing run, allowing the evaluation and discovery of novel small RNA molecules and the prediction of their functions.
Our small RNA sequencing combined with UMI technology recognizes PCR duplicates derived from a single molecule and delivers accurate, affordable sequencing data, leading to accurate estimates of quantitative small RNA expression.
Bacterial small RNA sequencing enables the detection of novel bacterial small RNA molecules, profiling of small RNA expression, prediction of small RNA targets, hence facilitating function and mechanism studies.
tRNA has abundant post-transcriptional modifications and plays a role in cDNA synthesis. tRNA sequencing provides an efficient and quantitative analysis of tRNA to sensitively detect tRNA mutations/isoforms/variants.
Our tRF&tiRNA sequencing service simultaneously detects tiRNA, tRF and miRNA in a given sample, allowing comprehensive expression profiling of tiRNA and tRF and biomarker discovery.
miRNA sequencing profiles microRNAs in any species, enabling the discovery of novel miRNAs and revelation of the importance of miRNAs linked to a wide spectrum of biological functions.
piRNAs are closely associated with the development of germ cells and axoneuron. We provide piRNA sequencing to capture all piRNAs in a sample, allowing for piRNA screening and research.
sdRNAs are found to be abnormally expressed in tumors, which suggests they are potential tumor biomarkers. sdRNA sequencing covers full-length sdRNAs, detect rare sdRNAs, and discover differentially expressed sdRNAs.
- Cancer transcriptomics
- Population genetics
- Pharmacogenomics applications
- Agricultural applications
- Illumian HiSeq 2500 / HiSeq 4000 / HiSeq X Ten / NovaSeq 6000 / NextSeq 500 / MiSeq
- PacBio RS II / Sequel
- Nanopore PromethION
- 10X Genomics
- Liu D, Mewalal R, Hu R, et al. New technologies accelerate the exploration of non-coding RNAs in horticultural plants. Horticulture Research, 2017, 4: 17031.
* For Research Use Only. Not for use in diagnostic procedures.