circRNA Microarray Service

The content of circRNAs in cells is extremely low, accounting for about 5-10% of linear RNAs. The amount of data that can be obtained by sequencing as junction reads for identifying circRNAs is even much lower than the requirements for accurate and quantitative data analysis. However, the detection accuracy of circRNA microarray is not affected by the low expression content of circRNA transcripts, which is more suitable for circRNA expression research.

Overview

Circular RNAs (circRNAs) are a class of non-coding RNAs formed by a special splicing mechanism, with closed-loop structures, and abundantly present in eukaryotic transcriptomes. The tissue specificity, disease specificity, timing specificity, and high stability of circRNA molecules make circRNAs have obvious advantages as biomarkers for clinical diseases. Recent studies have shown that circular RNAs function as miRNA sponges in different species, called competitive endogenous RNAs (ceRNAs), which can compete for binding to miRNAs. The interaction with disease-related miRNAs indicates that circRNAs play a very important role in the regulation of diseases. In addition, some intronic types of circular RNAs (circRNAs) facilitate the transcription of host genes.

CD Genomics' circRNA microarray has been rigorously verified to facilitate the systematic study of circRNAs under different physiological and pathological conditions. At the same time, we can annotate all differentially expressed circRNAs with miRNA target sites with high matching values, which will facilitate the study of the function of circRNAs as natural miRNA sponges.

Features

High Sensitivity & High Precision High Specificity Comprehensive Annotations Professional Bioinformatics
Can detect low expression of single copy in each cell. circRNA junction-specific microarray probes ensure the reliability of detection. Comprehensive circRNA targets and provides sufficient circRNA biological function annotations. High-quality lncRNA database, systematic and detailed annotation and functional analysis.

Project Workflow

Sample Preparation

1. Sample Preparation

RNA purification; quality assessment and quantification.

Library Preparation

2. Library Preparation

cDNA library construction, labeling, and labeling efficiency quality assessment.

Sequencing

3. Microarray Hybridization

The labeled probe was hybridized with a high-density microarray under standard conditions.

Data Analysis

4. Data Analysis

Preprocess and visualize results, and perform custom bioinformatics analysis.

In-depth data analysis:

We can provide detailed circRNA-miRNA interaction annotations, including miRNAs that circRNAs may bind to, and annotate potential binding sites, which will help researchers to further explore the functional role of circRNAs as miRNA adsorption sponges. We can also perform circRNA differential expression analysis and circRNA expression pattern analysis.

Sample Requirements

RNA samples or well-preserved tissue/cell specimens; RNA≥200 ng; Species: human, mouse, rat

Sample storage: Cell samples or fresh tissue pieces (cut into 5-10 mg pieces) can be treated with TRIzol or RNA protectant, frozen in liquid nitrogen, and stored at -80°C. RNA samples can be dissolved in ethanol or RNA-free ultrapure water, and stored at -80°C. Avoid repeated freezing and thawing during sample storage.

Shipping Method: The sample is stored in a 1.5 mL Eppendorf tube, sealed with sealing film. Shipments are generally recommended to contain 5-10 pounds of dry ice per 24 hours.

References:

  1. Li S, Teng S, Xu J, et al. Microarray is an efficient tool for circRNA profiling. Briefings in bioinformatics. 2019 Jul;20(4).
  2. Tang W, Fu K, Sun H, et al. CircRNA microarray profiling identifies a novel circulating biomarker for detection of gastric cancer. Molecular cancer. 2018 Dec;17(1).
* For Research Use Only. Not for use in diagnostic procedures.


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