Poly(A)-seq is a next-generation sequencing method that allows for transcriptome-wide profiling of polyadenylation sites. By selectively sequencing the 3' ends of mRNA molecules that have poly(A) tails, our Poly(A)-seq service provides a comprehensive view of mRNA stability and translation efficiency.
Poly(A) tails at the 3' end of eukaryotic messenger RNAs control mRNA stability and translation efficiency. Dynamic poly(A) tail regulation in the nucleus and cytoplasm has been implicated in various cellular and physiological processes. The next-generation sequencing provides ample opportunity to precisely delineate poly(A) tails. Various methods for profiling transcriptome-wide poly(A) sites were developed. Poly(A)-seq is a direct poly(A) sequencing method at the whole genome level. Therefore, Poly (A) -seq can be used as a potential tool to analyze the dynamic regulation process of mRNA turnover, degradation and translation, and it can also discover some unknown characteristics of RNA cracking and tailing.
Fig1 Overview of the function of mRNA poly(A) tails as master regulators of gene expression in the cytoplasm. (Passmore, 2022)
One of the unique features of Poly(A)-Seq library construction is its enrichment for mRNA. The poly(A) tails of mRNA molecules are specifically captured using oligo(dT) beads or other poly(A) binding methods, which allows for the analysis of gene expression and post-transcriptional regulation at the mRNA level. This results in higher specificity and sensitivity compared to other sequencing methods. Another speciality of Poly(A)-Seq library construction is its whole-genome coverage. It provides transcriptome-wide coverage of poly(A) sites, which allows for the detection of novel poly(A) sites that have not been previously annotated in reference genomes.
|High Reliability||Transcriptome-Wide||High Precision||Convenient|
|The correlation among samples within this method is better.||This method is a direct poly(A) sequencing method at the whole Transcriptome level.||Using this method accurate profiling of the complete poly(A) tails could be possible.||This method is a direct sequencing approach using Illumina sequencing platforms.|
Deliverable: FastQ, BAM, QC report, global profiling of poly(A), custom bioinformatics analysis.