Exploring ceRNA (including lncRNA, circRNA and Pseudogene) interactions can contribute to the functional roles of ncRNAs and their interplay with miRNAs and mRNAs. CD Genomics focuses on investigating the intricate interactions between long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and messenger RNAs (mRNAs), providing comprehensive insights into the regulatory networks in different research fields.
Overview
Interactions between long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and messenger RNAs (mRNAs) are mediated through a regulatory mechanism known as competing endogenous RNA (ceRNA) interaction. CeRNA interaction refers to the communication and crosstalk among these RNA species through the shared binding sites for miRNAs. It represents a complex network of interactions that modulate gene expression and play important roles in various biological processes and diseases.
We are committed to providing exceptional lncRNA-miRNA-mRNA interaction analysis services, delivering comprehensive insights into gene regulation and facilitating advancements in various fields of research based on our Sequencing Platform and advanced algorithms. Learn more about our lncRNA-RNA interaction prediction and miRNA-mRNA interactions analysis services and how they can benefit your research or project.
Features
High Compatibility
Integrated Analysis
Cutting-edge Algorithms
One-Stop Solution
Consider both coding and non-coding regions of the genome.
Combine experimental data with computational analysis to efficiently narrow down the relationships.
Identify lncRNAs, predict miRNA targets, and decipher the interactions between lncRNAs, miRNAs, and mRNAs.
One-stop solution from sample QC, library construction, to sequencing and data analysis.
Project Workflow
1. Sample Preparation
Quality assessment and quantification
2. Library Preparation
mRNA, small RNA and lncRNA library preparation
3. Sequencing
Illumina Nextseq/HiSeq
4. Data Analysis
Visualize and preprocess results, and perform custom bioinformatics analysis.
Service Pipeline
lncRNA Identification and Characterization: We employ sophisticated algorithms and databases to identify and classify lncRNAs in your dataset. This step is crucial for understanding their potential functions and regulatory roles. Learn more.
miRNA Target Prediction: Using cutting-edge bioinformatics tools, we predict the target mRNAs for the identified miRNAs. This step helps unravel the intricate interactions between miRNAs and mRNAs, revealing potential regulatory relationships. Learn more.
lncRNA–miRNA Interaction Analysis: We investigate the interactions between lncRNAs and miRNAs based on complementary binding and sequence complementarity. By deciphering these interactions, we can uncover the regulatory mechanisms underlying gene expression control. Learn more.
Functional Annotation and Pathway Analysis: To gain a comprehensive understanding of the biological implications of the identified lncRNA-miRNA-mRNA interactions, we perform functional enrichment analysis. This analysis helps uncover the involved biological processes, molecular functions, and cellular pathways.
Customized Reports and Visualization: We provide detailed reports summarizing the analysis results. These reports include interactive visualizations, such as network diagrams, heatmaps, and gene ontology plots, to aid in the interpretation and presentation of the findings.
Sample Requirements
RNA sample (concentration ≥ 200 ng/uL, quantity ≥ 1 ug)
1.8 ≤ OD260/280 ≤ 2.2, OD260/230≥2.0, RIN ≥ 6.5, 28S:18S≥1.0.
Please make sure that the RNA is not degraded. Sample Storage: RNA can be dissolved in ethanol or RNA-free ultra-pure water and stored at -80°C. RNA should avoid repeated freezing and thawing. Shipping Method: When shipping RNA samples, the RNA sample is stored in a 1.5 mL Eppendorf tube, sealed with a sealing film. Shipments are generally recommended to contain 5-10 pounds of dry ice per 24 hours. Deliverable: FastQ, BAM, coverage summary, QC report, custom bioinformatics analysis.
* For Research Use Only. Not for use in diagnostic procedures.
