Total RNA sequencing (RNA-seq) is a next-generation sequencing (NGS)-based method to capture a comprehensive view of the transcriptome. We provide total RNA-seq to help you analyze coding RNAs and multiple forms of ncRNA, including long noncoding RNA (lncRNA), small nuclear (snRNA), small nucleolar (snoRNA), and other ncRNA species.
RNA transcribed from DNA, is a single-stranded ribonucleic acid molecule and it plays a critical role in coding, decoding, expression and regulation of genes. There are many types of RNA, including mRNA, lncRNA, snRNA, snoRNA and other RNA species. In addition to mRNA, there are many types of non-coding RNAs (ncRNAs) that cannot be translated into proteins and function at the RNA level not protein level. Ribo-Zero ribosomal RNA reduction chemistry can maximize the percentage of uniquely mapped reads and minimize ribosomal contamination. Total RNA-Seq enables the analysis of both mRNA and a broad range of ncRNA species of interest combined the next-generation sequencing and proven ribosomal reduction library preparation chemistries, particularly suited for species whose mRNA may lack a poly-A tail, such as bacteria. It accurately measures gene and transcript abundance, and identify known and novel features of the transcriptome. We provide total RNA-Seq to discover the known and novel features, such as gene fusions, allele-specific expression, alternative transcripts, and the detection of novel transcripts in both coding and ncRNA species.
|Any Species||Transcriptome-Wide||Bioinformatics Analysis||Quality Assurance|
|This method can be applied to any species, from microorganisms to humans.||Profile all mRNAs, lncRNAs, snRNAs, snoRNAs, either known or unknown, in your biological sample.||Our integrated bioinformatics pipeline can be tailored to suit your project.||Sample QC, library QC, and reads QC using validated methods.|
Quality assessment and quantification
Ribosomal RNA depletion and fragmentation；250~300 bp insert cDNA Library
Illumina HiSeq; 150 bp paired end; >40 Million Reads
Visualize and preprocess results, and perform custom bioinformatics analysis
Sample storage: RNA can be dissolved in RNA Stable or RNA-free ultra-pure water and stored at -80°C. RNA should avoid repeated freezing and thawing.
Shipping Method: When shipping RNA samples, the RNA sample is stored in a 1.5 mL Eppendorf tube, sealed with a sealing film. Shipments are generally recommended to contain 5-10 pounds of dry ice per 24 hours.
Deliverable: FastQ, BAM, coverage summary, QC report, custom bioinformatics analysis.