Hi-C sequencing, as known as chromosome conformation capture sequencing, short for 3C-Seq, is a next-generation sequencing (NGS) based method for interpreting the interaction patterns among different DNA sequences, as well as providing high-resolution chromatin three-dimensional structure information.
Chromosomal conformation capture (3C) is changing our understanding of the spatial configuration of the genome. There are often two key points in speculating chromatin interactions: one is to generate high-resolution signals, and the other is to capture useful information from the background signals. 3C technology can be widely used in studying gene expression, chromatin spatial conformation, and chromatin interaction. 3C and its derived technologies follow the same basic rule: chromatin digestion and DNA reconnection, which gathers sequences that are spatially close together, even though they may be far apart on the genome. By analyzing the characteristics of these junctions, for example by sequencing, a detailed map of chromatin interactions can be obtained.
|Accuracy||Cost-efficient||Bioinformatics Analysis||One-stop Service|
|High resolution and highly fidelity conformation of chromatin.||Cost-efficient solutions to meet the needs of various research requirements.||Our integrated bioinformatics pipeline can be tailored to suit your project.||Provides one-stop service for library construction, sequencing, sample QC and data analysis.|
quality assessment and quantification.
Visualize and preprocess results, and perform custom bioinformatics analysis.
Cell sample: 0.5×106
RNA sample (concentration ≥ 1 ng/µL, quantity ≥ 5 µg)
1.8 ≤ OD260/280 ≤ 2.2, OD260/230 ≥ 2.0, RIN ≥ 6.5, 28S:18S ≥ 1.0.
Please make sure that the RNA is not degraded nor contaminated.
Sample storage: RNA can be dissolved in ethanol or RNA-free ultra-pure water and stored at -80°C. RNA should avoid repeated freezing and thawing.
Shipping Method: When shipping RNA samples, the RNA sample is stored in a 1.5 mL Eppendorf tube, sealed with sealing film. Shipments are generally recommended to contain 5-10 pounds of dry ice per 24 hours.
Deliverable: FastQ, BAM, coverage summary, QC report, full statistical analysis & alignments, custom analysis reports on customer request.