In the research of diseases such as cancer, it is not enough to know whether a gene is mutated or normal. It is also important to understand whether and how the gene is expressed. Transcriptome analysis of cancer samples is becoming increasingly important for understanding how changes in the expression of genetic variants lead to cancer and other diseases. The analysis of differential RNA expression provides researchers with a deeper understanding of the biological pathways and molecular mechanisms that regulate cell fate, development, and disease progression.
Monitoring changes in gene expression can be used to diagnose and characterize tumors. Related sequencing technologies include RNA-seq, small RNA sequencing, lncRNA sequencing, circRNA sequencing, targeted RNA sequencing, exosomal RNA sequencing, degradome sequencing, etc. Single-cell RNA sequencing allows us to obtain the whole transcriptome at the single-cell level. The development of single-cell sequencing technology provides a new way to understand the tumor more deeply, and it is expected to find a more effective method for the diagnosis and treatment of tumors.
Based on our next-generation sequencing and long-read sequencing platforms, CD Genomics provides a complete solution for cancer research. Our one-stop service can help you purify high-quality nucleic acids from a variety of research sample types, and use illumina next-generation sequencing, 10x Genomics single-cell sequencing, PacBio sequencing, and Nanopore direct RNA sequencing to provide accurate sequencing results. After bioinformatics analysis, Finally, provide customized reports and serve your scientific research work.
Our advantages
An abundance of sample handling experience
Enrichment and extraction of low-abundance transcripts
Multiple sequencing platforms to choose from
High accuracy of sequencing results
Comprehensive bioinformatics analysis and customized analysis
The issues that can be addressed with our RNA sequencing services
Differential cancer gene expression analysis
Cancer biomarker discovery and identification
Cancer heterogeneity and evolution research
Cancer drug resistance research
Cancer microenvironment and immunotherapy research
Neoantigen research
Drug research and development
Note: Our services are for research use only, not for disease diagnosis and treatment.
Sample types: various sample types, such as normal/tumor tissues, biological fluids, cfRNA
Data delivery: identification, quantitation, differential expression analysis, and other bioinformatics analyses
Service Workflow
Sequencing Platforms
| Illumina NextSeq/HiSeq/NovaSeq | 10x Genomics Single-Cell Sequencing | PacBio RS IL / Sequel | Nanopore |
|---|---|---|---|
| Cancer gene discovery Cancer gene expression Splice variation |
Single-cell cancer gene discovery Single-cell cancer gene expression Single-cell genetic structure Cancer cell heterogeneity |
Cancer gene expression Cancer transcript structures Cancer gene discovery Splicing / alternative splicing Transcription initiation RNA cleavage / adenylation |
Cancer gene expression Cancer transcript structures Cancer gene discovery Splicing / alternative splicing Transcription initiation RNA cleavage / adenylation RNA modification |
Bioinformatics Analysis Contents
| ANALYSIS CONTENTS | DETAILS |
|---|---|
| QC and Visualization | Filtering, trimming, optional error correction if possible, and visualization |
| Transcriptome Assembly | Mapping to known transcriptomes; de novo assembly and annotation to protein database; outcome evaluation; Transcript and isoform quantification |
| Functional Profiling | GO, DAVID, KEGG, TrEMBL, Pfam, InterPro, SignalP, Blast2GO; Molecular functions or pathways in which differentially expressed genes |
| Advanced Analysis | In-depth and advanced analyses: canonical pathways, mechanistic hypotheses, potential therapeutics, biomarkers, disease mechanisms |
Comprehensive Solutions to Accelerate Your Oncology Research
CD Genomics has a professional technical team and bio-analysis team, and has more than ten years of project experience in the sequencing field. We have a mature technical route, provide accurate and reliable data and deliver results that meet your requirements. We are committed to providing efficient, cost-effective, and high-quality sequencing services to help your research in oncology fields.
