In the research of diseases such as cancer, it is not enough to know whether a gene is mutated or normal. It is also important to understand whether and how the gene is expressed. Transcriptome analysis of cancer samples is becoming increasingly important for understanding how changes in the expression of genetic variants lead to cancer and other diseases. The analysis of differential RNA expression provides researchers with a deeper understanding of the biological pathways and molecular mechanisms that regulate cell fate, development, and disease progression.
Monitoring changes in gene expression is essential for studying tumor biology and gaining insights into molecular mechanisms underlying cancer progression. Common sequencing approaches include RNA-seq, small RNA sequencing, lncRNA sequencing, circRNA sequencing, targeted RNA sequencing, exosomal RNA sequencing, and degradome sequencing. These transcriptome-level analyses support in-depth exploration of gene regulation, non-coding RNA functions, and RNA processing events in tumor samples.
CD Genomics offers a comprehensive cancer research solution based on advanced next-generation sequencing (NGS) and long-read sequencing platforms. We provide end-to-end services, including high-quality nucleic acid extraction from various sample types, and utilize Illumina NGS, PacBio SMRT sequencing, and Oxford Nanopore direct RNA sequencing to deliver accurate transcriptomic data. Our bioinformatics pipelines ensure in-depth analysis, and all results are delivered with customized reports to support your research objectives.
Our advantages
An abundance of sample handling experience
Enrichment and extraction of low-abundance transcripts
Multiple sequencing platforms to choose from
High accuracy of sequencing results
Comprehensive bioinformatics analysis and customized analysis
The issues that can be addressed with our RNA sequencing services
Differential cancer gene expression analysis
Cancer biomarker discovery and identification
Cancer heterogeneity and evolution research
Cancer drug resistance research
Cancer microenvironment and immunotherapy research
Neoantigen research
Drug research and development
Note: Our services are for research use only, not for disease diagnosis and treatment.
Sample types: various sample types, such as normal/tumor tissues, biological fluids, cfRNA
Data delivery: identification, quantitation, differential expression analysis, and other bioinformatics analyses
Service Workflow
Sequencing Platforms
Illumina NextSeq/HiSeq/NovaSeq | PacBio RS IL / Sequel | Nanopore |
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Cancer gene discovery Cancer gene expression Splice variation |
Cancer gene expression Cancer transcript structures Cancer gene discovery Splicing / alternative splicing Transcription initiation RNA cleavage / adenylation |
Cancer gene expression Cancer transcript structures Cancer gene discovery Splicing / alternative splicing Transcription initiation RNA cleavage / adenylation RNA modification |
Bioinformatics Analysis Contents
ANALYSIS CONTENTS | DETAILS |
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QC and Visualization | Filtering, trimming, optional error correction if possible, and visualization |
Transcriptome Assembly | Mapping to known transcriptomes; de novo assembly and annotation to protein database; outcome evaluation; Transcript and isoform quantification |
Functional Profiling | GO, DAVID, KEGG, TrEMBL, Pfam, InterPro, SignalP, Blast2GO; Molecular functions or pathways in which differentially expressed genes |
Advanced Analysis | In-depth and advanced analyses: canonical pathways, mechanistic hypotheses, potential therapeutics, biomarkers, disease mechanisms |
Comprehensive Solutions to Accelerate Your Oncology Research
CD Genomics has a professional technical team and bio-analysis team, and has more than ten years of project experience in the sequencing field. We have a mature technical route, provide accurate and reliable data and deliver results that meet your requirements. We are committed to providing efficient, cost-effective, and high-quality sequencing services to help your research in oncology fields.