microRNA Expression Analysis

CD Genomics is a Next-generation sequencing (NGS) and bioinformatics solution provider with solid expertise in genomics and analysis of high-throughput sequencing data. We provide integrative microRNA solutions from microRNA target prediction to microRNA expression profiling, as well as other customized bioinformatics analysis. Please do not hesitate to discuss your project.

Overview

microRNAs (miRNA) are ~22 nucleotide long endogenous RNA molecules that resemble small interfering RNAs (siRNAs) of the RNA interfering (RNAi) pathway. They play a vital role in post-transcriptional regulation of target genes by mRNA cleavage, direct translational repression and /or mRNA destabilization. Although thousands of miRNAs have been identified using high-throughput sequencing technologies. miRNAs are known to play important roles in organismal development, immunity and cancer. Aberrant alteration of the miRNA profile has been linked to the development of certain diseases. Correcting changed miRNAs by their mimics or inhibitors has been developed as potential therapeutic approaches. Emerging studies are focusing on miRNA therapeutic strategy for diagnostics and treatment of disease.

CD Genomics provides next-generation sequencing (NGS) approaches coupled with bioinformatics analysis for miRNA expression analysis, contributing to understanding the dynamic level and complicated functions of miRNAs. Information on global miRNA expression and differentially expressed miRNAs is very important in elucidating mechanisms underlying disease onset and progression and discovering novel diagnostic, prognostic and therapeutic targets. microRNA sequencing coupled with advanced bioinformatics pipelines enables fast and accurate profiling of miRNAs and identification of candidates for novel directions in therapy development. We can help you to get the most out of your NGS experiments by developing comprehensive data analysis strategies.

Features

Rich Experience Accurate Profiling Integrative Solution Flexibility
Rich experience in library preparation, microRNA sequencing and bioinformatics analysis. Utilize recognized NGS approaches and computational tools for accurate expression profiling. Provide integrative microRNA solution from expression profiling, target prediction to functional analysis. Flexible to your needs with strong expertise in experimental operation and data analysis.
microRNA Expression Analysis

Data Analysis Workflow

In-depth data analysis:

  • Data preprocessing and filtering
  • miRNA expression profiling
  • Differential expression analysis
  • miRNA target prediction
  • GO functional analysis
  • KEGG pathway analysis
  • Statistical analysis

Sample Requirements

RNA sample (concentration ≥ 200 ng/uL, quantity ≥ 4 ug), 1.8 ≤ OD260/280 ≤ 2.2, OD260/230 ≥ 2.0, RIN ≥ 6.5, 28S:18S ≥ 1.0.
Please make sure that RNA is not significantly degraded.

Sample storage: The sample should be stored at -80°C. Avoid repeated freezing and thawing.

Shipping Method: When shipping the sample, it is stored in a 1.5 mL Eppendorf tube, sealed with a sealing film. Shipments are generally recommended to contain 5-10 pounds of dry ice per 24 hours.

Deliverable: FastQ, BAM, coverage summary, QC report, GO enrichment histogram, GO terms DAG (directed acyclic hierarchical graph), and KEGG enrichment scatter plot, and other designated report.

Reference:

  1. Kovanda A, Leonardis L, Zidar J, et al. Differential expression of microRNAs and other small RNAs in muscle tissue of patients with ALS and healthy age-matched controls. Scientific reports, 2018, 8(1): 1-15.
* For Research Use Only. Not for use in diagnostic procedures.


Inquiry

  • Verification code
logo
Copyright © 2022 CD Genomics. All rights reserved.