Third generation sequencing (TGS), also called long-read sequencing, emerged in 2011, provided by Oxford Nanopore Technologies (ONT) and Pacific Biosciences (PacBio). TGS is a single molecular and long read lengths technology, but with low accuracy. Advantages of TGS include: 1) Longer read lengths have more information than the short reads; 2) TGS can be used to resolve structural variants and repetitive regions; 3) TGS can be used to improve existing reference genomes; 4) TGS is good for sequencing new genomes; 5) TGS can be used to explore epigenetic modifications. There are two kinds of RNA TGS, single molecule real-time (SMRT) full-length RNA-sequencing and nanopore direct RNA sequencing. (SMRT) full-length RNA-sequencing based on sequencing by synthesis. RNA sample was reverse-transcription to cDNA, as DNA polymerase incorporated labeled dNTPs into the single-stranded cDNA template, the fluorescence pulses was recorded. Nanopore direct RNA sequencing using nanopore inserted in an electrical resistant membrane. As the RNA fragment pass through the nanopore, the electrical current change was measured, indicating a specific RNA sequence. Nanopore direct RNA sequencing allows ultra-long reads, the longest fragment is close to 1 million bp. It's a direct, real-time analysis technology. These two technologies can help you to identify novel genetic diseases, also can be used to target genome editing by the combination of TGS with other technologies, such as single-cell RNA sequencing or NGS.
(SMRT) full-length sequencing (Edi J, 2009)
Nanopore direct sequencing (Schatz MC, 2017)
We provide TGS based (SMRT) full-length RNA-sequencing and nanopore direct RNA sequencing services for deciphering transcriptomics, which can be used to identify new transcripts and alternative splicing events, resolve structural variants and repetitive regions with low alignment and mapping errors during library construction. We provide ultra-long reads sequencing for accurate analysis with a short sequencing time, help you sequence new transcriptomics and improve existing reference transcriptomics.
Explore how our RNA third-generation sequencing services help your analysis of long RNA fragments, identify new transcripts, alternative splicing events and help you sequence new transcriptomics.