Long-Read RNA Sequencing

Overview

Third generation sequencing (TGS), also called long-read sequencing, emerged in 2011, provided by Oxford Nanopore Technologies (ONT) and Pacific Biosciences (PacBio). TGS is a single molecular and long read lengths technology, but with low accuracy. Advantages of TGS include: 1) Longer read lengths have more information than the short reads; 2) TGS can be used to resolve structural variants and repetitive regions; 3) TGS can be used to improve existing reference genomes; 4) TGS is good for sequencing new genomes; 5) TGS can be used to explore epigenetic modifications. There are two kinds of RNA TGS, single molecule real-time (SMRT) full-length RNA-sequencing and nanopore direct RNA sequencing. (SMRT) full-length RNA-sequencing based on sequencing by synthesis. RNA sample was reverse-transcription to cDNA, as DNA polymerase incorporated labeled dNTPs into the single-stranded cDNA template, the fluorescence pulses was recorded. Nanopore direct RNA sequencing using nanopore inserted in an electrical resistant membrane. As the RNA fragment pass through the nanopore, the electrical current change was measured, indicating a specific RNA sequence. Nanopore direct RNA sequencing allows ultra-long reads, the longest fragment is close to 1 million bp. It's a direct, real-time analysis technology. These two technologies can help you to identify novel genetic diseases, also can be used to target genome editing by the combination of TGS with other technologies, such as single-cell RNA sequencing or NGS.

(SMRT) full-length sequencing (SMRT) full-length sequencing (Edi J, 2009)

Nanopore direct sequencing Nanopore direct sequencing (Schatz MC, 2017)

We provide TGS based (SMRT) full-length RNA-sequencing and nanopore direct RNA sequencing services for deciphering transcriptomics, which can be used to identify new transcripts and alternative splicing events, resolve structural variants and repetitive regions with low alignment and mapping errors during library construction. We provide ultra-long reads sequencing for accurate analysis with a short sequencing time, help you sequence new transcriptomics and improve existing reference transcriptomics.

Features

  • Single-base resolution, flexible and high throughput
  • Identification of novel diseases
  • Direct, real-time analysis of long RNA fragments
  • Identify new transcripts and alternative splicing events
  • Resolve structural variants and repetitive regions
  • Low alignment and mapping errors during library construction

Service Portfolio

Explore how our RNA third-generation sequencing services help your analysis of long RNA fragments, identify new transcripts, alternative splicing events and help you sequence new transcriptomics.

(SMRT) full-length RNA-sequencing

We provide long-read sequencing for accurate analysis of structural variation, delivering a more complete view of genetic diversity.

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Nanopore Direct RNA-sequencing

We provide ultra-long reads sequencing for sequencing new transcriptomics and improving existing reference transcriptomics.

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Long-Read Epitranscriptomics Sequencing

We utilize long-read sequencing platforms to achieve RNA modifications detection across the entire transcriptome at single-base resolution.

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References:

  1. Xiao T, Zhou W. The third generation sequencing: the advanced approach to genetic diseases. Translational Pediatrics, 2020, 9(2): 163–173.
  2. Edi J, et al. Real-time DNA sequencing from single polymerase molecules. Science, 2009, 323(5910): 133-8.
  3. Schatz MC. Nanopore sequencing meets epigenetics. Nature Methods, 2017, 14: 347-348.
* For Research Use Only. Not for use in diagnostic procedures.


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