Term-seq is a service provided by your company that is designed to accurately map 3' ends of bacterial RNA transcripts. This service is particularly useful in bacteria because bacterial mRNAs lack the polyA tail that is present in eukaryotic RNA transcripts, which makes it challenging to map 3' ends using traditional sequencing methods.
Term-seq works by directly sequencing the exposed RNA 3' ends in bacteria, which allows for the precise mapping of RNA termini in a highly quantitative manner. This provides a genome-wide map of RNA termini, which can be used to better understand the mechanisms of transcription termination in bacteria.
In addition to its utility in mapping RNA termini, Term-seq can also be used to identify novel ribo-regulators, which are RNA molecules that can regulate gene expression. This provides synthetic biologists with additional tools for controlling gene expression in bacteria and can also provide insights into the mechanisms of transcription termination. Overall, Term-seq is a powerful tool for studying gene expression in bacteria and can be used to uncover new regulatory mechanisms and potential therapeutic targets.
The library construction of Term-seq involves a series of steps that result in the generation of a cDNA library from bacterial RNA transcripts, which is then sequenced using high-throughput sequencing platforms. The resulting sequence reads are processed using bioinformatics tools to accurately map the 3' end termini of RNA transcripts and quantify their expression levels. This provides a highly quantitative and precise map of RNA termini in bacteria, which can be used to study gene expression regulation and transcription termination mechanisms.
|Specialization||Transcriptome-Wide||High Precision||High Sensitivity|
|This method is specially developed for bacteria.||Profile all transcriptional termination, either known or unknown, in your bacteria.||This method can map RNA termini to the single-base resolution across the bacterial genome.||This method accurately reconstitutes the exact 3' end termini in a highly quantitative manner.|
Deliverable: FastQ, BAM, QC report, Integrative view of the bacterial transcriptome (Multi-layered RNA sequencing data), Folding energy of RNA, Custom bioinformatics analysis.