miRNA-mRNA Interactions Analysis

MicroRNAs (miRNAs) are key regulators of gene expression and their dysregulation has been associated with various diseases. Understanding the interactions between miRNAs and messenger RNAs (mRNAs) is crucial for unraveling gene regulatory networks and discovering diagnostic and prognostic markers. CD Genomics offers a comprehensive miRNA-mRNA interaction analysis service to investigate these relationships using deep sequencing and advanced bioinformatics techniques.


miRNAs are small RNA molecules that bind to target mRNAs, leading to mRNA degradation or inhibition of protein translation. Multiple miRNAs often act cooperatively to regulate a single gene, while a single miRNA can target multiple genes. These complex interactions pose challenges for fully characterizing miRNA-mRNA interaction networks. Functional characterization of these interactions requires specialized computational platforms and analysis of RNA-Seq data.

Our miRNA-mRNA Integrated Analysis Service combines state-of-the-art computational algorithms and advanced bioinformatics techniques to analyze high-throughput sequencing data, such as small RNA-seq and mRNA-seq data. By integrating miRNA and mRNA analysis, we offer a holistic view of gene regulation networks, enabling the identification of key regulatory interactions and potential therapeutic targets. We also offer Integrative lncRNA Solutions and microRNA Target Prediction services.


High Compatibility Integrated Analysis Cutting-edge Algorithms One-Stop Solution
Identify microRNA binding regions in target mRNAs. Combine experimental data with computational analysis to efficiently narrow down the relationships between miRNAs and mRNAs. Stay up-to-date with the latest research and methodologies in miRNA-mRNA analysis. One-stop solution from sample QC, library construction, to sequencing and data analysis.

Project Workflow

Sample Preparation

1. Sample Preparation

Quality assessment and quantification

Library Preparation

2. Library Preparation

cDNA and small RNA library preparation


3. Sequencing


Data Analysis

4. Data Analysis

Visualize and preprocess results, and perform custom bioinformatics analysis.

Bioinformatics Analysis Pipeline

Sample Requirements

RNA sample (concentration ≥ 200 ng/uL, quantity ≥ 1 ug)
1.8 ≤ OD260/280 ≤ 2.2, OD260/230≥2.0, RIN ≥ 6.5, 28S:18S≥1.0.
Please make sure that the RNA is not degraded.
Sample Storage: RNA can be dissolved in ethanol or RNA-free ultra-pure water and stored at -80°C. RNA should avoid repeated freezing and thawing.
Shipping Method: When shipping RNA samples, the RNA sample is stored in a 1.5 mL Eppendorf tube, sealed with a sealing film. Shipments are generally recommended to contain 5-10 pounds of dry ice per 24 hours.
Deliverable: FastQ, BAM, coverage summary, QC report, custom bioinformatics analysis.

* For Research Use Only. Not for use in diagnostic procedures.

Research Areas
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