Exosomal lncRNA Sequencing

Exosomal lncRNA sequencing is a method that combines barcoded cDNA library preparation and next-generation sequencing technology to comprehensively detect long RNA sequences from exosomes. We offer this unparalleled end-to-end service to deliver the highest quality sequencing data and accelerate your exosomal lncRNA research. Exosomal lncRNA sequencing can help you analyze all RNA molecules longer than 200 nt in length, including mRNA and lncRNA, and circular RNA (circRNA), from exosomes at one time.

Overview

Exosomes are cell-derived vesicles that are widely distributed in many fluids, including urine, blood, ascites, and cultured medium of cell cultures. Exosomes play an important role in disease transmission and intercellular communication. Exosomes contain various proteins, lipids and nucleic acids such as lncRNAs, microRNAs (miRNAs), mRNAs, circular RNAs (circRNAs) and other non-coding RNAs (ncRNAs). Exosomal lncRNA expression patterns may be distinct from those on the cellular level. As lncRNAs are found to be closely related to cancer, exosomal RNAs, especially long non-coding RNA, may perform important functions in cell-cell communication and serve as potential biomarkers for diagnosing and monitoring human diseases.

With decades of experience in exosomal lncRNA sequencing, we can generate high-quality, reproducible and reliable exosomal lncRNA libraries and deliver accurate data and comprehensive data analysis using the advanced Illumina next-generation sequencing (NGS) and well-accepted computational tools. Our bioinformatics team provides both lncRNA and mRNA information in a single sequencing run, enabling prediction of lncRNA-mRNA association network. Exosomal lncRNA-Seq service can be used to find the molecular marker for liquid biopsy and reveal the mechanism of disease development.

Features

Quality Assurance Transcriptome-Wide Bioinformatics Analysis One-Stop Solution
Sample QC, library QC, and read QC using validated methods. Profile all lncRNAs and mRNAs, either known or unknown, in exosomes. Provide in-depth data analysis based on professional the bioinformatics team. One-stop solution from sample QC, library construction, to sequencing and data analysis.

 

Sample Preparation

1. Sample Preparation

QC and quantification;
rRNA and DNA depletion

Library Preparation

2. Library Preparation

250-300 bp insert directional cDNA library

Sequencing

3. Sequencing

Illumina platforms, PE 150 bp;
≥ 12 G Raw Data / Sample

Library Preparation

4. Data Analysis

Visualize and preprocess results, and perform custom bioinformatics analysis.

Bioinformatics Analysis Pipeline

Exosomal lncRNA Sequencing

In-depth data analysis:

  • lncRNA target prediction
  • GO analysis
  • KEGG analysis
  • Coding-non-coding gene co-expression network (CNC network)
  • lncRNA-miRNA-mRNA network
  • Prediction of lncRNA binding sites
  • lncRNA-GSEA

Sample Requirements

RNA sample (concentration ≥ 1 ng/uL, quantity ≥ 2 ug)

1.8 ≤ OD260/280 ≤ 2.2, OD260/230≥2.0, RIN ≥ 6.5, 28S:18S ≥ 1.0.

Please make sure that the RNA is not significantly degraded.

Sample storage: RNA can be dissolved in ethanol or RNA-free ultra-pure water and stored at -80°C. RNA should avoid repeated freezing and thawing.

Shipping Method: When shipping RNA samples, the RNA sample is stored in a 1.5 mL Eppendorf tube, sealed with sealing film. Shipments are generally recommended to contain 5-10 pounds of dry ice per 24 hours.

Deliverable: FastQ, BAM, coverage summary, QC report, custom bioinformatics analysis.

References:

  1. Yang, J, Wei, F, Schafer, C, et al. Detection of tumor cell-specific mRNA and protein in exosome-like microvesicles from blood and saliva. PloS one, 2014, 9(11): e110641.
  2. Mohankumar S, Patel T. Extracellular vesicle long noncoding RNA as potential biomarkers of liver cancer. Brief Funct Genomics, 2016, 15(3):249-56.
* For Research Use Only. Not for use in diagnostic procedures.


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