RNAs can be modified post-transcriptionally with dynamic and reversible modifications such as N6-methyladenosine (m6A). RNA methylation dynamically regulates the processing, export, translation, and stability of RNA molecules, thereby affecting biological and pathological processes such as differentiation and cellular response to stress and tumorigenesis. Epitranscriptomics refers to the field that studies RNA modifications. The majority of RNA modifications involve the addition of a methyl group to certain positions on the RNA such as m6A, N1-methyladenosine (m1A), 5-methylcytosine (m5C) and 2'-O-methylation. Sometimes, acetylation also occurs on RNA molecules. Next-generation sequencing (NGS)-based methods, either relying on antibody enrichment or third-generation sequencing, have been developed to map post-transcriptional RNA modification and explore their functions, We provide a full range of epitranscriptomics services to investigate RNA modifications using NGS or long-read sequencing (Oxford nanopore sequencing and PacBio SMRT sequencing). We can help you detect many forms of RNA modifications including m7G, m3C, m1A, 5mC, 5hmC, m6A, m6Am, acetylation, and 2'-O-methylation.
Features
Single-base resolution and high throughput
Cover mRNA, lncRNA, pri-miRNA, tRNA, rRNA and other RNAs
Rigorous quality control and fast turn-around times
Professional bioinformatics analysis in an efficient and customizable way
Service Portfolio
Explore how our epitranscriptomics services help researchers map RNA modifications and explore their pathways.
N7-methylguanosine (m7G) is an essential modification at the 5' cap of eukaryotic mRNA. We provide m7G-MeRIP sequencing to map m7G and reveal its distribution features.
We provide a deep sequencing-based service for the simultaneous profiling of 7-methylguanosine (m7G) and 3-methylcytidine (m3C) in RNA at single nucleotide resolution.
Cytosines can be methylated in the form of 5mC (m5C) and subsequently oxidized to generate 5hmC. We provide RNA BS-seq, m5C-RIP-seq, and hMeRIP-seq for profiling 5mC, or 5hmC, or both.
acRIP-Seq can be used to depict a transcriptome-wide map of N4-acetylcytidine (ac4C) and reveal its roles in RNA stability, alternative splicing, gene expression regulation, and protein synthesis.
2'-O-methylation is a common RNA modification, mostly found in rRNA, snRNA. 2'-O-methylation sequencing enables a transcriptome-wide mapping of this modification.
Pseudouridylation sequencing is a NGS-based method to comprehensively detect global maps of pseudouridylation, a post-transcriptional modification in various molecules.
Illumian HiSeq 2500 / HiSeq 4000 / HiSeq X Ten / NovaSeq 6000 / NextSeq 500 / MiSeq
PacBio RS II / Sequel
Nanopore PromethION
10X Genomics
References:
Peer E, Rechavi G, Dominissini D. Epitranscriptomics: regulation of mRNA metabolism through modifications. Current opinion in chemical biology, 2017, 41: 93-98.
Shafik A, Schumann U, Evers M, et al. The emerging epitranscriptomics of long noncoding RNAs. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms, 2016, 1859(1): 59-70.
* For Research Use Only. Not for use in diagnostic procedures.
