CD Genomics provides Comprehensive Epitranscriptomics Services to unlock the RNA modification code with cutting-edge sequencing. Through our bioinformatic capacity, we can help you with the potential of RNA methylation in your studies and accelerate discoveries in gene regulation, RNA stability, and beyond.
Overview
The world of RNA modifications is a fascinating and ever-expanding realm, with scientists having identified over 170+ distinct RNA modifications in various cellular ribosomal RNAs (rRNAs). These modifications go beyond the traditional four RNA residues, revealing a complex tapestry of chemical alterations that contribute to the intricate regulation of cellular processes. Among these, methylation modifications take center stage, constituting more than 60% of all known RNA chemical modifications.
RNA Methylation Modifications
5-Methylcytosine (m5C)
N1-Methyladenosine (m1A)
N6-Methyladenosine (m6A)
N7-Methylguanosine (m7G)
2'-O-Methylation
Role
Implicated in RNA stability, translation efficiency, and gene expression regulation.
Involved in tRNA stability and fidelity during translation.
The most abundant RNA modification in eukaryotic cells, influences mRNA stability, processing, and translation.
Enhances mRNA stability and is associated with translational efficiency.
Implicated in RNA stability and structure, as well as regulation of RNA function.
Significance
A key player in the epitranscriptomic landscape, influencing diverse cellular processes.
Essential for maintaining translation accuracy and preventing errors.
Central to post-transcriptional gene regulation and cellular responses.
A common modification contributing to mRNA function and regulation.
Plays a role in RNA stability, structure, and function.
Adopts carefully optimized experimental procedure, achieving high efficiency and specificity.
A broad spectrum of RNA methylation modifications, including N6-methyladenosine (m6A), 5-methylcytosine (m5C), and more.
Integrate RNA methylation data with other omics data such as DNA methylation, histone modification, and gene expression, enabling comprehensive systems biology studies.
One-stop solution, covering everything from library construction and sequencing to sample quality control and data analysis.
Project Workflow
1. Sample Preparation
Quality assessment and quantification.
2. Library Preparation
3. Sequencing
Illumina HiSeq; PE 50/75/100/150.
4. Data Analysis
Visualize and preprocess results, and perform custom bioinformatics analysis.
Bioinformatic Analysis
Methylation Site Identification: Detect and annotate RNA methylation sites in your samples.
Differential Methylation Analysis: Compare methylation profiles between different conditions or samples.
Functional Enrichment Analysis: Gain insights into the biological significance of methylated RNA molecules.
Visualization: We provide interactive visualizations to facilitate data interpretation.
Sample Requirements
Sample Starting Volume：can be reduced to 10-20 μ, with a minimum of 1 μg of total RNA. You are encouraged to consult with our technical team to discuss specific requirements and ensure compatibility with their samples.
Sample Types：is compatible with a diverse range of sample types, including animals, plants, cells, and tissues.
Please make sure that the RNA is not significantly degraded.
Sample storage: RNA can be dissolved in ethanol or RNA-free ultra-pure water and stored at -80°C. RNA should avoid repeated freezing and thawing.
Shipping Method: When shipping RNA samples, the RNA sample is stored in a 1.5 mL Eppendorf tube, sealed with sealing film. Shipments are generally recommended to contain 5-10 pounds of dry ice per 24 hours.
