Polysome Profiling (Polysome-seq)

At CD Genomics, we are dedicated to providing you with groundbreaking insights into gene expression and translation regulation within cells. Our state-of-the-art technology allows you to delve deep into the world of polysomes, the dynamic complexes responsible for protein synthesis.

Overview of Polysome Profiling

The number of ribosomes bound to the mRNA reflects the translation efficiency of the gene and detecting mRNA translation efficiency is of great significance for the study of eukaryotic gene expression regulation. Polysome profiling takes advantage of the large sedimentation coefficient of ribosomes to separate polysomes by sucrose density gradient centrifugation. The more ribosomes bound to an mRNA, the faster the sedimentation rate during gradient centrifugation. Polysome profiling is increasingly used at the gene expression level of eukaryotes in life activities such as growth, disease, apoptosis, and tumorigenesis.

Our comprehensive Polysome Profiling Service covers the entire process, from experimental design to data analysis, enabling you to unravel the intricacies of cellular mechanisms with unprecedented precision.

CD Genomics polysome profiling serviceCD Genomics polysome profiling service

Polysome Profiling vs. Ribo-Seq: Both are complementary techniques that can be chosen based on the research questions and goals. Polysome profiling provides insights into translation efficiency and regulation at a broader scale, while Ribo-Seq offers detailed information about ribosome positions and potential alternative translation events.

Contact us to learn more about how our Polysome Profiling and Ribo-Seq services can advance your research endeavors.

Project Workflow

Sample Preparation

1. Sample Preparation

Quality assessment and quantification.

Library Preparation

2. Library Preparation

Bridge PCR amplification.


3. Sequencing

Illumina sequencing, 2*150 sequencing mode.

Data Analysis

4. Data Analysis & Delivery

Visualize and preprocess results, and perform custom bioinformatics analysis.

Data Analysis

  • Raw data QC
  • Read alignment
  • Transcript assembly analysis
  • Gene expression analysis
  • Alternative splicing
  • Function analysis
  • Gene set enrichment analysis

Sample Requirements

When the sample is cultured to an appropriate time point, it is necessary to add cycloheximide (CHX, the final concentration is 100 μg/mL) to the medium for treatment. Store at -80°C after nitrogen quick freezing. (Please consult for specific experiments and sample types)

Experiment period: 11-13 weeks

DEMO result:

DEMO result

* For Research Use Only. Not for use in diagnostic procedures.

Research Areas
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